SIXTH FRAMEWORK PROGRAMME
PRIORITY 1
LIFE SCIENCES, GENOMICS AND BIOTECHNOLOGY FOR HEALTH
DIAMONDS PROJECT
Contract no: LSHG-CT-2004-512143
EU Deliverable
D 1.1
Optimised synchronisation protocol for yeasts, Arabidopsis, human cells
Date: 6th February 2006
Version 3
Main Partner responsible: Dirk Inze (VIB)
Other partners involved: Kristian Helin (BRIC), Jurg Bahler (Sanger), Soeren Brunak (CBS)
This deliverable is meant to share the synchronisation protocols developed and currently used by DIAMONDS consortium members with the research community. The different protocols are listed below:
Saccharomyces cerevisiae:
Arabidopsis thaliana:
- D1.1.1a: synchronisation protocol (12.01.06)
- D1.1.1b: growth of large batches (12.01.06)
These protocols are modifications of protocols originally described by Menges et al.:
Menges
M, de Jager SM, Gruissem W, Murray JA.
Global analysis of the core cell cycle regulators of Arabidopsis identifies
novel genes, reveals multiple and highly specific profiles of expression and
provides a coherent model for plant cell cycle control. Plant J. 2005 Feb;41(4):546-66.
Menges
M, Hennig L, Gruissem W, Murray JA.
Genome-wide gene expression in an Arabidopsis cell suspension. Plant Mol Biol.
2003 Nov;53(4):423-42.
Menges
M, Murray JA.
Cryopreservation of transformed and wild-type Arabidopsis and tobacco
cell suspension cultures. Plant J. 2004 Feb;37(4):635-44.
Menges
M, Murray JA.
Synchronous Arabidopsis suspension cultures for analysis of cell-cycle
gene activity. Plant J. 2002 Apr;30(2):203-12.
Schizosaccharomyces pombe:
Human cells: